肉毒杆菌毒素(简称肉毒素)是迄今所知最强的毒物,它选择地作用于突触前,阻遏神经肌肉传递。肉毒中毒虽非常见多发病,但至今在世界各地,包括我国的某些地区在内仍时有发生。科学家们长期以来致力于寻找实际可用的肉毒中毒解毒药,但除特异的抗毒血清外至今尚无一确有疗效的药物。在先前的工作中我们发现,从中药楝属植物皮提取的一个三萜化合物——川楝素也是一个选择的接头前阻遏剂。李培忠等的实验表明,川楝素不但可以保护接受致死量肉毒注射的小鼠,而且能治愈接受致死剂量肉毒中毒的部分恒河猴。本工作则利用离体神经肌肉标本进一步证实了川楝素的抗肉毒作用。 Botulinum toxin (botulinum toxin for short) is by far the strongest known poison, it selectively acts on the presynaptic, blocking neuromuscular transmission. Although botulism is a common and frequently occurring disease, it has still occurred in various parts of the world, including some parts of our country. Scientists have long been dedicated to finding practical botulism detoxification drugs, but to date no specific and effective anti-drug drugs have been available. In our previous work, we found that toosendanin, a triterpenoid extracted from the genus Apiaceae, is also a selective pre-linker repressor. Experiments such as Li Peizhong have shown that toosendanin not only protects mice receiving lethal botulinum injections but also can cure some rhesus monkeys receiving lethal doses of botulism. This work is the use of isolated neuromuscular specimens to further confirm the anti-botulinum toxin effect.