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目的探讨玻璃体内植入胰高血糖素类肽-1(GLP-1)缓释珠对大鼠视网膜神经节细胞的保护作用。设计实验研究。研究对象SPF级Sprague-Dawley(SD)雄性大鼠25只。方法将25只大鼠随机分为2组,实验组13只,对照组12只。实验组大鼠右眼玻璃体内植入4个GLP-1缓释珠,对照组右眼玻璃体内注入4μl复方氯化钠。GLP-1缓释珠直径600μm,内含3000个整合了GLP-1基因的人骨髓间充质干细胞,外被致密的藻酸盐外膜,以确保GLP-1产物可顺利释放而不引起免疫排斥。玻璃体内注射均在右眼视神经夹伤后立即进行。视神经夹伤后第23天用3%荧光金从双侧上丘做逆行标记,第28天取双眼球标本做视网膜铺片并在荧光显微镜下拍摄照片,采用人工双盲法进行视网膜神经节细胞计数。主要指标视网膜神经节细胞密度以及视网膜神经节细胞存活率。结果视网膜神经节细胞密度实验组与对照组分别为(2113±474)/mm~2和(1734±424)/mm~2,两组之间的差异有统计学意义(t=-2.111,P=0.046)。视网膜神经节细胞存活率实验组与对照组分别为(74±18)%和(57±16)%,两组之间的差异有统计学意义(t=-2.451,P=0.022)。结论GLP-1缓释珠玻璃体内植入后对视神经夹伤大鼠视网膜神经节细胞具有保护作用,可以提高视网膜神经节细胞存活率。
Objective To investigate the protective effect of intravitreal GLP-1 sustained release beads on rat retinal ganglion cells. Design experiment research. Twenty-five SPF Sprague-Dawley (SD) male rats were studied. Methods Twenty-five rats were randomly divided into two groups: experimental group (n = 13) and control group (n = 12). In the right eye of experimental group, four GLP-1 sustained-release beads were implanted in the right eye and 4μl of compound sodium chloride in the right eye of the control group. GLP-1 Sustained-release beads with a diameter of 600μm contain 3000 human bone marrow mesenchymal stem cells integrated with GLP-1 gene and outer dense alginate outer membrane to ensure smooth release of GLP-1 without causing immunity Exclusion. Intravitreal injections were performed immediately after the right optic nerve crush. On the 23rd day after optic nerve crush, retrograde labeling was performed on both sides of the superior colliculus with 3% fluorescent gold. On the 28th day, double-eyeball specimens were used for retinal spreading and photographs were taken under a fluorescence microscope. The retinal ganglion cells count. The main indicators of retinal ganglion cell density and retinal ganglion cell survival rate. Results The density of retinal ganglion cells was (2113 ± 474) / mm ~ 2 and (1734 ± 424) / mm ~ 2 in the experimental group and the control group, respectively. The difference between the two groups was statistically significant (t = -2.111, P = 0.046). The survival rate of retinal ganglion cells was (74 ± 18)% and (57 ± 16)% in experimental and control groups, respectively. The difference between the two groups was statistically significant (t = -2.451, P = 0.022). Conclusion GLP-1 sustained-release beads have the protective effect on retinal ganglion cells injured by optic nerve crush in rats after vitreous implantation, which can improve the survival rate of retinal ganglion cells.