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采用菌液浇施的方法接种10株桉树内生菌于尾巨桉幼苗。接种30 d后,用GFS3000气体交换—荧光测量系统进行光合作用各项指标的测定。光响应研究表明,不同菌株的最大净光合速率变化范围为9.31-16.37μmol.m-.2s-1;不同菌株的光饱和点变化范围为1 007.23-1 408.65μmol.m-.2s-1;初始量子效率的变化范围为0.023-0.034;暗呼吸速率变化范围为0.01-1.97。CO2响应研究表明,不同菌株的最大净光合速率变化范围为14.45-36.0μmol.m-.2s-1;不同菌株的CO2饱和点变化范围为1 449.49-2 000μmol.mol-1。不同菌株CO2补偿点变化范围为39.72-203.79μmol.mol-1。温度响应研究表明,不同菌株最大净光合速率不尽相同,变化范围为6.69-15.30μmol.m-.2s-1。通过对接种后植株的光响应、CO2响应和温度响应的研究,得出菌株7在各个响应中,光合作用能力均较大程度地高于对照,表明其对促进植株的光合作用效能具有很大的功用。
10 strains of Eucalyptus endophytes were inoculated on E. urophylla seedlings by using the method of fungus pouring. Thirty days after inoculation, the GFS3000 gas exchange-fluorescence measurement system was used to measure photosynthesis. Light response studies showed that the maximum net photosynthetic rate of different strains ranged from 9.31-16.37μmol.m-2s-1. The light saturation point of different strains ranged from 1007.23-1 to 408.65μmol.m-2s-1. The initial quantum efficiency ranged from 0.023 to 0.034 and the dark respiration rate varied from 0.01 to 1.97. CO2 response studies showed that the maximum net photosynthetic rate of different strains ranged from 14.45-36.0μmol.m-2s-1; the CO2 saturation point of different strains varied from 1 449.49-2 000μmol.mol-1. The CO2 compensation points of different strains ranged from 39.72-203.79μmol.mol-1. Temperature response studies showed that the maximum net photosynthetic rate of different strains vary, ranging from 6.69-15.30μmol.m-.2s-1. Through the study of light response, CO2 response and temperature response of inoculated plants, it was concluded that the photosynthesis ability of strain 7 was higher than that of the control in all responses, indicating that it had a great effect on the photosynthesis of plants The function.