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目的探讨肺炎克雷伯菌环磷酸腺苷受体蛋白(CRP)调控子对细菌毒力及生物膜形成的影响。方法 2012年8月到2014年2月,选择120株肺炎克雷伯菌进行CRP调控子的PCR阳性表达检测,同时选择哌拉西林、哌拉西林+他唑巴坦、阿米卡星、头孢他啶、头孢曲松、头孢哌酮+舒巴坦、左氧氟沙星等进行耐药性与生物膜形成实验。结果 PCR检测CRP调控子阳性率为33.3%。而CRP调控子阳性株对于头孢曲松、左氧氟沙星、阿米卡星与头孢他啶有更高的耐药性,与CRP调控子阴性株对比差异有统计学意义(P<0.05)。2组生物被膜培养组CRP调控子表达量为0.28±0.04,普通液相培养组CRP调控子表达量为0.08±0.05,2组对比差异有统计学意义(P<0.05)。结论肺炎克雷伯菌CRP调控子的阳性表达有利于降低细菌毒力与促进生物膜形成,从而提高相关抗菌药物的耐药性。
Objective To investigate the effects of the Klebsiella pneumoniae cyclic adenosine monophosphate receptor protein (CRP) regulator on the bacterial virulence and biofilm formation. Methods From August 2012 to February 2014, 120 strains of Klebsiella pneumoniae were selected for PCR positive detection of CRP. At the same time, we selected piperacillin, piperacillin + tazobactam, amikacin, ceftazidime , Ceftriaxone, cefoperazone + sulbactam, levofloxacin and other drug resistance and biofilm formation experiments. Results The positive rate of CRP regulator detected by PCR was 33.3%. The CRP positive control strains were more resistant to ceftriaxone, levofloxacin, amikacin and ceftazidime than those with CRP negative regulator (P <0.05). The expression of CRP regulators in the biofilm culture group was 0.28 ± 0.04, that in the normal liquid culture group was 0.08 ± 0.05, there was significant difference between the two groups (P <0.05). Conclusion The positive expression of CRP regulator of Klebsiella pneumoniae is favorable to reduce the bacterial virulence and promote the formation of biofilm, and thus enhance the drug resistance of the related antibacterials.