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[目的]研究古巴蝇人工繁殖技术及其对二点螟的寄生能力。[方法]通过人工接种试验,研究了不同蝇蛆与大蜡螟幼虫接种比例对古巴蝇生长发育的影响;通过室内低温储藏试验,研究了不同低温对古巴蝇蛹羽化率的影响;通过室内、田间比较试验,研究了不同生态条件(湿度、阳光)对古巴蝇蛹羽化率的影响以及古巴蝇对二点螟的寄生能力。[结果]蝇蛆与大蜡螟幼虫的最佳接种比例为3:1和4:1,古巴蝇产蛹量分别为279和327;10℃温度条件下进行短期贮藏对古巴蝇蛹的羽化率影响较小,但贮藏时间不宜超过15d;室内、田间寄生试验结果表明,古巴蝇对二点螟的寄生能力较强,寄生率达33.3%~50.0%;在甘蔗拔节期和成熟期寄生率分别为47.8%和41.7%,苗期几乎不能寄生。[结论]该研究结果为人工大规模繁殖古巴蝇及其田间释放技术提供了科学依据。
[Objective] The research aimed to study the artificial breeding techniques of Cuba flies and their parasitism to the rice borer. [Method] The effects of inoculation ratio of different maggot larvae on the growth and development of the guinea flies were studied by artificial inoculation test. The effects of different hypothermia on the eclosion rate of the pupae were studied by indoor low temperature storage test. Field experiments were conducted to study the effects of different ecological conditions (humidity and sunshine) on the eclosion rate of pupae in Cuba and the parasitism of the fly on the borer. [Result] The optimal inoculation rates of maggot larvae and larvae were 3: 1 and 4: 1, respectively. The pupae of Cuban flies were 279 and 327, respectively. The emergence rate of pupae in Cuban fly pupae at 10 ℃ But the storage time should not exceed 15d. The results of indoor and field parasitization test showed that the parasitism rate of Cuban fly was 33.3% ~ 50.0%, and the parasitism rate of jointing stage and mature stage of sugar cane were respectively 47.8% and 41.7%, respectively, the seedling stage can hardly parasitize. [Conclusion] The results of this study provided a scientific basis for artificial large-scale propagation of Cuban flies and their field release technology.