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目的 :探讨基因重组转化生长因子α 绿脓杆菌外毒素融合蛋白 (TGFα PE4 0 ,简称TP4 0 )对人肺腺癌细胞生长的导向抑制作用。方法 :用免疫组化LSAB法检测人肺腺癌SPC A 1细胞表皮生长因子受体 (EGFR)的表达 ,用结晶紫染色法和 3H 亮氨酸掺入法检测TP4 0对SPC A 1细胞生长及蛋白质合成的抑制 ,用过量EGF竞争拮抗TP4 0的细胞毒作用。结果 :SPC A 1细胞免疫组化显示出大量棕黄色的EGFR阳性染色。TP4 0浓度为 1～10 0ng/ml时 ,对SPC A 1细胞生长及蛋白质合成呈剂量依赖性抑制。过量EGF能完全拮抗TP4 0的细胞毒作用。结论 :人肺腺癌SPC A 1细胞能高水平地表达EGFR ,重组TP4 0对SPC A 1细胞生长具有较强的抑制作用 ,作用部位为细胞的EGFR。
Objective: To investigate the inhibitory effect of TGFα PE4 0 (TP4 0) on the growth of human lung adenocarcinoma cells. Methods: The expression of epidermal growth factor receptor (EGFR) in human lung adenocarcinoma SPC A 1 cells was detected by immunohistochemical LSAB method. The growth of SPC A 1 cells was detected by crystal violet staining and 3H-leucine incorporation assay And protein synthesis, with excessive EGF competition antagonistic TP4 0 cytotoxicity. RESULTS: Immunohistochemical SPC A 1 cells showed a large number of tan-positive EGFR-positive staining. When TP40 concentration was 1 ~ 100ng / ml, the growth and protein synthesis of SPC A 1 cells were inhibited in a dose-dependent manner. Excess EGF completely antagonized the cytotoxic effect of TP40. Conclusion: Human lung adenocarcinoma SPC A 1 cells can express EGFR at a high level. Recombinant TP 4 0 has a strong inhibitory effect on the growth of SPC A 1 cells. The site of action is EGFR.