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目的通过化学方法制成动物核性白内障模型,探讨相对能量复合指数(RECP)与角膜内皮细胞活性的关系。方法将5种化学物质注入晶状体,以透过黑白条纹的清晰程度判断晶状体混浊程度并分级。然后将实验眼球分为6组(Ⅰ组为对照组;Ⅱ-Ⅵ为实验组),行标准超声乳化白内障摘除手术。手术后立即取下角膜,作锥蓝-茜素红联合染色标本和扫描电镜标本。结果晶状体内注入甲醛、冰醋酸、无水乙醇、丙酮和苯扎溴铵均能形成晶状体混浊,其中:冰醋酸致晶状体混浊能力最弱,无水乙醇、丙酮次之,甲醛、苯扎溴铵最强。当RECP≤90时,锥蓝-茜素红双重染色和扫描电镜均表明角膜内皮细胞活性好,其形态和细胞联接均无改变。当RECP=120时,角膜内皮细胞形态尚正常,但是细胞联结和胞膜部分破坏。当RECP=150时,角膜内皮细胞严重损伤。结论用化学方法制作核性白内障模型供过渡训练使用是可行的。当RECP超过某一数值(>90)时,即与角膜内皮细胞的活性成负相关。
OBJECTIVE: To establish animal model of nuclear cataract through chemical methods and to explore the relationship between relative energy recombination index (RECP) and corneal endothelial cell activity. Methods Five chemicals were injected into the lens to determine the degree of lens opacity and to classify it through the clarity of the black and white stripes. Then, the experimental eyeballs were divided into 6 groups (group Ⅰ as control group and group Ⅱ-Ⅵ as experimental group) underwent standard phacoemulsification cataract extraction. Immediately after surgery, the cornea was removed for coned blue-alizarin red combined staining and scanning electron microscopy. RESULTS: Lens opacification could be induced by the injection of formaldehyde, glacial acetic acid, absolute ethanol, acetone and benzalkonium bromide in the lens. Among them, glacial acetic acid caused the weakest lens opacification, followed by ethanol, acetone, formaldehyde and benzalkonium bromide The strongest When RECP was less than or equal to 90, double staining of cones blue - alizarin red and scanning electron microscopy showed that corneal endothelial cells were active and their morphology and cell junctions had no change. When RECP = 120, the morphology of corneal endothelial cells is still normal, but cell junctions and cell membrane partial destruction. Corneal endothelial cells are severely damaged when RECP = 150. Conclusion It is feasible to make the nuclear cataract model by chemical method for transitional training. When RECP exceeds a certain value (> 90), it is negatively correlated with corneal endothelial cell activity.