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用 RAPD方法分析了苍术不同居群间的亲缘关系。选取 10个 10碱基的随机引物对苍术的 10个居群共83个个体进行扩增 ,获得 10 0个 DNA片断。用 RAPD istance软件计算了不同居群间的 Jaccard遗传距离 ,得距离矩阵 ,分别用单联法、全联法、类平均法作聚类图 ,结果表明 ,该 10个居群可划分为 3个类群 :即类群 1,大别山类群(Ta) ;类群 2 ,南苍术类群 (Wu、L u、Hu、Xu、Ca、L i) ;类群 3,北苍术类群 (Tt、Da、L ao)。在这 3个类群中 ,南、北苍术之间的遗传距离相对较近 ,大别山类群与南、北苍术之间的距离则较远。根据本试验的结果及前人关于苍术精油成分差异的聚类结果 ,作者认为大别山类群比较特殊
RAPD was used to analyze the genetic relationship among different populations of Atractylodes lancea. Ten random primers with 10 bases were used to amplify 10 individuals in 10 populations of Atractylodes lucidum and obtain 10 0 DNA fragments. The RAPD istance software was used to calculate the Jaccard genetic distance among different populations and to obtain the distance matrix. The single cluster method, the all-joint method, and the class average method were used as the clustering maps. The results showed that the 10 populations could be divided into 3 groups. Groups: Group 1, Dabie Mountains (Ta), Group 2, Southern Atractyloids (Wu, Lu, Hu, Xu, Ca, Li), Taxus 3, Atractylodes (Tt, Da, Lao). Among these three groups, the genetic distance between the northern and southern atractive arteries is relatively close, and the distance between the Dabie Mountain group and the northern and southern atractive techniques is relatively far. Based on the results of this experiment and previous clustering results on the differences in the essential oil composition of Atractylodes chinensis, the author believes that the Dabie Mountains group is rather special.