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目的探讨线粒体DNA(mtDNA)5178A/C,10398A/G多态性与中国新疆维吾尔族长寿老人的关联性。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)的分析方法,将165例新疆维吾尔族长寿老人分为百岁组(≥100岁)65例,长寿组(91~99岁)100例和112例对照组(≤70岁)比较进行研究。结果在百岁组中mtDNA 5178A等位基因频率高于对照组(P=0.0029),而百岁组和长寿组10398G等位基因的频率显著高于对照组(P<0.005)。百岁组和长寿组中mtDNA 5178A/10398G基因型(A-G单倍型)组合明显高于对照组。结论线粒体DNA5178A和10398G等位基因与新疆维吾尔族长寿相关。5178A和10398G等位基因及A-G基因型组合(A-G单倍型)为长寿的保护因素。
Objective To investigate the association of mitochondrial DNA (mtDNA) 5178A / C and 10398A / G polymorphisms with the elderly Uygur in Xinjiang in China. Methods 165 age-related Uyghur longevity people in Xinjiang were divided into three groups: one hundred centenarians (≥100 years old), 65 cases of longevity group (91-99 years old) ) 100 cases and 112 cases of control group (≤70 years) compared to study. Results The frequency of mtDNA 5178A allele in the centenarian group was higher than that in the control group (P = 0.0029), while the frequency of the 10398G allele in the centenarian group and the longevity group was significantly higher than that of the control group (P <0.005). The combination of mtDNA 5178A / 10398G genotype (A-G haplotype) in centenarian group and longevity group was significantly higher than that in control group. Conclusion Mitochondrial DNA5178A and 10398G alleles are associated with the Uighur longevity in Xinjiang. The 5178A and 10398G alleles and the A-G genotype combination (A-G haplotype) are protective factors of longevity.