Objective:To study the expression of microRNA-130b(miR-l 30b) in children acute promyelocytic leukemia(APL) and its role for regulating PTEN expression.Methods:A total of SO children APL marrow tissues and IS normal marrow tissues between January and December in 2012 were collected into our study.The expression of miR-l30 b in APL and normal marrow tissues were detected by quantitative real-time polymerase chain reaction.MiR-l30 b inhibitor was transfected into HL-60 cells.Cell Counting Kit-8 assay and flow cytometry were used to measure cell proliferation and apoptosis.respectively.The expression of PTEN,a potential target of miR-130 b,and its downstream genes,Bcl-2 and Box,in transformed cells were detected by quantitative real-time polymerase chain reaction and western-blot Results:The expression of miR-l30 b was significantly higher in children APL marrow tissues than in normal marrow tissues(P<0.05).Down-regulation of miR-1 30 b could significantly suppress cell proliferation and induce apoptosis in HL-60 cells(P<0.05).PTEN expression was upregulated when miR-130 b was knocking-down(P<0.05).As downstream genes of PTEN,the expression of Bcl-2 and Box were regulated as well.Conclusions:MiR-130 b is overexpressed in children APL marrow tissues and associated with cell growth.MiR-130 b may promote children APL progression by inducing cell proliferation and inhibiting apoptosis. Objective: To study the expression of microRNA-130b (miR-I 30b) in children acute promyelocytic leukemia (APL) and its role for regulating PTEN expression. Methods: A total of SO children APL marrow tissues and IS normal marrow tissues between January and December in 2012 were collected into our study. The expression of miR-l30b in APL and normal marrow tissues were detected by quantitative real-time polymerase chain reaction. MiR-l30b inhibitor was transfected into HL-60 cells. Cell Counting Kit- 8 assay and flow cytometry were used to measure cell proliferation and apoptosis. Promptly. The expression of PTEN, a potential target of miR-130 b, and its downstream genes, Bcl-2 and Box, in transformed cells were detected by quantitative real- time polymerase chain reaction and western-blot Results: The expression of miR-l30 b was significantly higher in children APL marrow tissues than in normal marrow tissues (P <0.05). Down-regulation of miR- and were downregulated when miR-130b was knocking-down (P <0.05) .As downstream genes of PTEN, the expression of Bcl-2 and Box were regulated as well .Conclusions: MiR-130 b is overexpressed in children APL marrow tissues and associated with cell growth. MiR-130 b may promote children APL progression by inducing cell proliferation and inhibiting apoptosis.