[目的]测定盒子草粗多糖的抑菌活性。[方法]采用滤纸片抑菌圈实验法测定盒子草多糖抑菌效果;进而采用试管法测定盒子草多糖的最低抑菌浓度(MIC)。[结果]盒子草多糖对实验菌有明显的抑制作用,其中对黑曲霉和枯草杆菌抑制作用最强,MIC=0.25 mg/ml,而对青霉和马青霉作用很弱,MIC=4 mg/ml。[结论]盒子草多糖具有一定的抑菌活性。 [Objective] The research aimed to determine the antibacterial activity of crude polysaccharide from Boxtide. [Method] The antibacterial effect of polysaccharides was determined by the inhibition zone test of filter paper. The minimum inhibitory concentration (MIC) of polysaccharides was determined by in vitro test. [Result] The results showed that the polysaccharide from the box had a significant inhibitory effect on the experimental bacteria, of which the inhibitory effect on Aspergillus niger and Bacillus subtilis was the strongest, MIC = 0.25 mg / ml, while the effect on Penicillium and Penicillium sp. Was weak with MIC = 4 mg / ml. [Conclusion] Box-grass polysaccharide has antibacterial activity.