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目的:研究细胞基因工程人α干扰素(rhIFN-α_1)对体外7种流感病毒感染的MDCK细胞以及对甲型流感病毒鼠肺适应株PR_8引起的小鼠肺炎的对抗作用。方法:接种7种病毒液(H_1N_1,H_2N_2,H_3N_3,B型,C型,A_1,B分离株)于MDCK细胞中,小鼠用PR_8病毒液滴鼻,观察rhIFN-α_1的抗病毒作用。结果:rhIFN-α_1对7种病毒液的最小有效浓度分别为12.5,25,50,25,12.5,25和12.5kU·L~(-1);在MDCK细胞上对7种病毒液的感染治疗指数为8×10~3,4×10~3,2×10~3,4×10~3,8×10~3,4×10~3和8×10~3;抑制指数为3.6,4.7,3.5,3.3,3.9,4.6和3.5 ;rhIFN-α_1能有效地抑制流感病毒的细胞内复制,却不能直接杀伤病毒;rhIFN-α_1对小鼠病毒肺炎有抑制作用,使肺组织炎症,纤维间质增生明显改善,降低病毒滴度,延长生命率为94.2％-132.7％,肺指数抑制率为14.8％-37.4％。结论:rhIFN-α_1对流感病毒的增殖有抑制作用,并且能够改善流感病毒引起的小鼠肺炎症状。
AIM: To investigate the antagonism of rhIFN-α 1 induced by recombinant human interferon-alpha (IFN-α 1) on MDCK cells infected by seven influenza viruses in vitro and mouse pneumonia induced by PR_8 strain of influenza virus. Methods: Seven strains of virus (H_1N_1, H_2N_2, H_3N_3, B, C, A_1, B) were inoculated into MDCK cells. The mice were challenged with PR_8 virus to observe the antiviral effect of rhIFN-α_1. Results: The minimum effective concentrations of rhIFN-α 1 against the seven virus solutions were 12.5, 25, 50, 25, 12.5, 25 and 12.5 kU · L -1, respectively. The infection of 7 virulent viruses on MDCK cells The indexes were 8 × 10 ~ 3, 4 × 10 ~ 3, 2 × 10 ~ 3, 4 × 10 ~ 3, 8 × 10 ~ 3, 4 × 10 ~ 3 and 8 × 10 ~ , 3.5,3.3,3.9,4.6 and 3.5; rhIFN-α_1 can effectively inhibit the influenza virus intracellular replication, but can not directly kill the virus; rhIFN-α_1 inhibition of viral pneumonia in mice, lung inflammation, inter-fiber Hyperplasia significantly improved, reducing the virus titer, to extend the life rate of 94.2% -132.7%, lung index inhibition rate of 14.8% -37.4%. Conclusion: rhIFN-α_1 can inhibit the proliferation of influenza virus and improve the symptoms of influenza virus-induced mouse pneumonia.