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对我国登革 3型病毒 80 2株基因组进行全序列测定 ,为了解其基因组结构与功能的关系提供依据 .根据登革 3型病毒H87株的序列设计并合成引物 ,应用RT PCR和RACE法 ,对 80 2株基因组RNA进行扩增、克隆测序后获得我国登革 3型病毒广西株基因组序列 .该株病毒基因组全长10 696nt ,不含poly(A)尾 ,4种碱基数分别为A :3 4 3 7,C :2 2 15,G :2 773 ,U :2 2 71.包含一个读码框架 ,自 95至 10 2 67位 ,共 10 170个碱基 ,编码 3 3 90个氨基酸 ,5′和 3′非编码区长度分别为 94nt和4 3 2nt.与H 87株比较 ,核苷酸和氨基酸序列同源性均在 99%以上 ,有 2 8个碱基发生改变 ,其中 2 6个碱基突变发生在读码框架内 ,碱基转换 18个 ,颠换 10个 ;碱基突变引起 14个氨基酸的改变 .80 2株与H87株病毒的基因组全序列同源性高 ,变异度小 .
The whole genome of 80 dengue virus 3 strains was sequenced in order to provide a basis for understanding the relationship between genomic structure and function.According to the sequence of dengue 3 virus H87 strain, primers were designed and synthesized by RT PCR and RACE, A total of 80 69 strains of genomic RNA were amplified and cloned and sequenced to obtain the genomic sequence of Dengue 3 virus Guangxi strain.The genome of the strain was 10 696 nt in total length without poly (A) tail, and the four bases were A : 3 4 3 7, C: 2 2 15, G: 2 773, U: 2 2 71. A reading frame consisting of 10 to 170 bases from 95 to 10267, encoding 3390 amino acids , And the 5 ’and 3’ non-coding regions were 94 nt and 432 nt, respectively. Compared with the H 87 strain, the nucleotide and amino acid sequence homologies were over 99% with 2 8 bases changed, of which 2 6 base mutations occurred in the reading frame, base conversion 18, transversion 10; base mutation caused by 14 amino acid changes.802 strains and H87 strains of the virus genome sequence homology, high degree of variation small .