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目的:探讨不规则抗体检测中抗-M抗体的检测及其对血型鉴定和交叉配血试验的影响。方法:检测IgG性质抗体用2-巯基乙醇(2-Me)应用液破坏IgM型抗-M;推断抗-M抗体用自制NN型的多人份A细胞核B细胞制备反定型标准红细胞;处理冷凝集用37℃生理盐水洗涤4次后做正定型,洗涤后的红细胞加等量自身血清,在4℃吸收后做反定型。结果:在20 018例住院患者中,检出139例同种不规则抗体,其中17例存在抗-M抗体,抗-M抗体占不规则抗体的比率为12.23%(17/139)。NN型的反定型红细胞正反定型一致。选择与受血者ABO同型的MM型或MN型红细胞交叉配血,卡式配血结果不合;选择与受血者ABO同型的NN型红细胞交叉配血,盐水和抗人球蛋白介质均不凝集,卡式配血结果主次侧均相合。结论:患者血清中存在抗-M抗体影响血型定型及配血,筛选相应红细胞输注无输血不良反应。
Objective: To investigate the detection of anti-M antibody in irregular antibody test and its effect on blood type identification and cross-match blood test. Methods: Antibodies of IgG were detected by using 2-mercaptoethanol (2-Me) solution to destroy IgM anti-M. The anti-M antibody was inferred to be made into reverse-typed erythrocytes by using self-made NN multi-cell A cell nuclear B cells. Set with 37 ℃ saline for 4 times after positive stereotypes, washed red blood cells plus the same amount of self-serum, done after 4 ° C anti-stereotypes. RESULTS: Of the 20 018 hospitalized patients, 139 cases of the same kind of irregular antibodies were detected, of which 17 cases had anti-M antibodies and the anti-M antibodies accounted for 12.23% (17/139). NN-type anti-stereotypes consistent red and red blood cells. Select the same type of blood recipient ABO MM or MN type red blood cells cross-matching blood, the card with the results of the mismatch; choose ABO blood donors with the same type of NN-type red blood cells cross-matching blood, saline and anti-human globulin medium are not agglutinated , Card-type blood results are primary and secondary side are consistent. Conclusion: The presence of anti-M antibody in serum of patients affected blood typing and blood distribution, and the corresponding red blood cells were screened for no transfusion adverse reactions.