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Objective:To establish a rat model of diabetic ulcer infected by Staphylococcus aureus and Escherichia coli, and provide a suitable animal model for experimental research of diabetic ulcer. Methods: Forty male SD rats were randomly divided into five groups: general ulcer group, diabetic ulcer group, diabetic ulcer treatment group, model group, and model treatment group. After modeling, take local skin tissue for HE staining.Detection of high-sensitivity C-reactive protein, fibroblast growth factor, tumor necrosis factor-α, advanced glycosylation products, interleukin-1β, interleukin-6, and nitric oxide in rat serum by enzyme-linked immunosorbent Level, and record the wound healing time, and evaluate the stability and practicability of the model. Results:The healing time of ulcer in the model group was significantly delayed compared with other groups (p ＜0.001). The serum H-CRP levels in the model group and the model treatment group were higher than those in the common ulcer group, the diabetic ulcer group, and the diabetic ulcer treatment group (p ＜0.05). After healing, the levels of H-CRP, bFGF, AGEs, TNF-α, IL-1β, IL-6, and NO in the serum of the diabetic ulcer group were higher than those in the common ulcer group (all p ＜0.05); The levels of TNF-α, IL-1β, IL-6, and NO were higher than those in other groups (all p ＜0.05); HCRP, AGEs, TNF-α, IL-1β, IL-6, and NO levels in the serum of the model treatment group They were all higher than those in diabetic ulcer treatment group (all p ＜0.05). Conclusion: This model is in line with the local environment of infectious diabetic ulcers and provides a reliable tool for the study of diabetic infections.