For more than 20 years, since it was first published in 1983, the Laboratory biosafety manual has provided practical guidance on biosafety techniques for use in laboratories at all levels. Good microbiological technique and appropriate use of biosafety equipment by well trained staff remain the fundamental elements of laboratory biosafety. However, globalization, significant advances in technology, the emergence of new diseases and serious threats arising from the deliberate misuse and release of microbiological agents and toxins have necessitated a review of current procedures. For this new edition, therefore, the manual has been extensively revised and expanded. For more than 20 years, since it was first published in 1983, the Laboratory biosafety manual has provided practical guidance on biosafety techniques for use in laboratories at all levels. Good microbiological technique and appropriate use of biosafety equipment by well trained staff remain the fundamental elements However, globalization, significant advances in technology, the emergence of new diseases and serious dangers arising from the deliberate misuse and release of microbiological agents and toxins have necessitated a review of current procedures. For this new edition, therefore, the manual has been extensively revised and expanded.