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目的了解口腔鳞状细胞癌细胞周期蛋白E(cyclin E)表达与中心体扩增相关性,探讨其中心体扩增的可能分子机制。方法正常口腔黏膜组织12例,不同分化程度的口腔鳞状细胞癌46例石蜡包埋组织,采用间接免疫荧光双重染色(γ-微管蛋白单克隆抗体及细胞角蛋白多克隆抗体)观察口腔鳞状细胞癌中心体扩增状况;采用免疫组织化学(SABC法)检测相应组织cyclin E蛋白表达情况.分析cyclin E蛋白表达与中心体扩增之间的相关性。结果中心体扩增可见于80.4%(37/46)口腔鳞状细胞癌组织中,而cyclin E蛋白过表达可在65.2%(30/46)的口腔鳞状细胞癌组织中见到;中心体扩增发生率在cyclin E阳性组为90.0%(27/30),而在cyclin E蛋白阴性组为10/16,两组间差异有统计学意义(X~2=5.014,P<0.05);Spearman相关分析显示中心体扩增与cyclin E蛋白阳性表达间存在相关关系(r=0.330,P<0.05);绝对危险度分析OR值为5.400(1.130,25.809)。结论肿瘤细胞中心体循环调控可能是一个多因素参与的复杂过程,cyclin E蛋白表达的高调作为危险因素之一可能在口腔鳞状细胞癌中心体扩增中起一定作用。
Objective To investigate the correlation between the expression of cyclin E and centrosome amplification in oral squamous cell carcinoma and to explore the possible molecular mechanism of centrosome amplification. Methods 12 cases of normal oral mucosa tissues and 46 cases of oral squamous cell carcinoma of different differentiation degree were paraffin-embedded, and the oral squamous cell carcinoma was observed by indirect immunofluorescence double staining (γ-tubulin monoclonal antibody and cytokeratin polyclonal antibody) The status of cyclin E protein was detected by immunohistochemistry (SABC method), and the correlation between cyclin E protein expression and centrosome amplification was analyzed. Results The centrosome amplification was found in 80.4% (37/46) oral squamous cell carcinoma tissues, while the overexpression of cyclin E protein was found in 65.2% (30/46) oral squamous cell carcinoma tissues. The centrosome The amplification rate was 90.0% (27/30) in cyclin E positive group and 10/16 in cyclin E negative group (X 2 = 5.014, P <0.05). Spearman correlation analysis showed that there was a correlation between centrosome amplification and the positive expression of cyclin E protein (r = 0.330, P <0.05). The OR of absolute risk analysis was 5.400 (1.130, 25.809). Conclusion The regulation of tumor cell centrosome circulation may be a complicated process involving many factors. The high-profile cyclin E protein expression may play a role in the amplification of centrosome in oral squamous cell carcinoma.