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目的 :研究天花粉蛋白（ trichosanthin,TCS）对胃癌细胞的作用，探讨其治疗胃癌的可能作用机制。方法 :采用生长曲线、集落形成实验、 MTT法、电镜、 TUNEL染色和流式细胞仪等技术，研究 TCS对胃癌细胞 MKN－ 45的增殖抑制和诱导凋亡作用。结果： TCS能够明显抑制胃癌细胞 MKN－ 45的生长和集落形成， 1μ g/ml和 0.1μ g/ml TCS作用 MKN－ 45后，细胞集落形成率分别为 (6.63± 1.31)％和 (14.68± 1.27)％，明显低于对照组的 (23.67± 2.76)％ (P
Objective: To study the effect of trichosanthin (TCS) on gastric cancer cells and explore its possible mechanism of action in gastric cancer. METHODS: Growth inhibition, colony formation assay, MTT assay, electron microscopy, TUNEL staining, and flow cytometry were used to study the effects of TCS on proliferation inhibition and apoptosis induction of gastric cancer cell MKN-45. RESULTS: TCS could significantly inhibit the growth and colony formation of gastric cancer cell MKN-45. After MKN-45 was treated with 1 μg/ml and 0.1 μg/ml TCS, the colony formation rate was (6.63± 1.31)% and (14.68±), respectively. 1.27)%, significantly lower than the control group (23.67± 2.76)% (P