论文部分内容阅读
肿瘤基因扩增是肿瘤基因激活的一种方式。而表皮生长因子受体基因在结构上作为v-erb-B的同源基因;在功能上其产物与src基因家族产物有相同的作用。因此,它的扩增与否与肿瘤的发生发展密切相关。因为肿瘤基因的激活,能导致肿瘤基因产物的异常表达。而表皮生长因子受体基因及表达产物的异常,又可能是细胞恶性增殖、低度分化的重要原因之一。 为了比较中国人常见的肿瘤细胞株表皮生长因子受体基因在DNA水平上的扩增状态,本研究选用了九种不同的肿瘤细胞株,用分子杂交的方法进行了研究。
Tumor gene amplification is a way of tumor gene activation. Epidermal growth factor receptor genes are structurally homologous to v-erb-B and functionally have the same function as the src gene family of products. Therefore, its amplification or not is closely related to the occurrence and development of tumors. Because tumor gene activation, can lead to the abnormal expression of tumor gene products. The abnormalities of epidermal growth factor receptor genes and their expression products may be one of the important reasons for malignant proliferation and poor differentiation of cells. In order to compare the amplification status of epidermal growth factor receptor gene at the DNA level in Chinese common tumor cell lines, nine different tumor cell lines were selected and studied by molecular hybridization method.