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为了探寻云南高原粳稻白叶枯病菌间毒素的差异及毒素与病菌致病性的关系,本研究选用致病性差异较大的3个云南高原粳稻白叶枯病菌株,采用乙酸乙酯法提取其毒素,用水稻幼苗浸根法和种子发芽抑制法测定毒素粗提物的生物活性,并用TLC法分析毒素组分及组分含量的差异。结果表明3个菌株单细胞产毒素量与菌株的致病性强弱成正相关;供试的3个菌株,无论其致病力强弱,其产生的毒素只要有足够的量,都能抑制水稻种子发芽,也能使水稻幼苗萎蔫,且毒素浓度越高,作用越明显;菌株间毒素组分和组分含量存在差异,一些特异的组分是否与其致病性有关,需要深入研究。
In order to explore the relationship between the isolates of toxins and the pathogenicity of the toxins to the bacterial blight in Yunnan Plateau, three strains of the japonica rice bacterial leaf blight with different pathogenicity were selected and extracted by ethyl acetate Their toxins were tested for the bioactivity of the crude toxin by using rice seedling immersion method and seed germination inhibition method. The contents of toxins and their components were analyzed by TLC. The results showed that three strains of single-cell toxin production and the pathogenicity of the strains are positively correlated; tested three strains, regardless of its virulence, the toxins produced by it as long as there is enough amount, can inhibit rice Seed germination can also make rice seedlings wilting, and the higher the concentration of toxins, the more obvious role; strains of toxins and components of the composition of the difference, some specific components whether its pathogenicity needs further study.