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采用荧光探剂乙酰乙酸盐 2 ,7 二氯氢化荧光素 ( 2 ,7-dichlorofluorescindiacetate ,D 399)和碘化丙啶 (PropidiumIodide ,PI)双标记 ,激光共聚焦扫描显微镜 (Laserconfocalscanningmicro scope ,LCSM)断层光切扫描观察脑缺血缺氧损伤时脑片脂质过氧化水平和细胞坏死程度 .L NG 硝基 精氨酸 (L NG nitro arginine ,L NNA)、N 乙酰半胱氨酸 (N acetylcysteine ,NAC)预保护可显著减轻大鼠脑片缺氧和 /或复氧损伤 ,说明一氧化氮 (NO)和其他自由基在脑缺氧复氧损伤中起重要作用
The fluorescence confocal laser scanning microscopy (LCSM) was used to detect the fluorescence intensity of the fluorescence emission spectrum of the macromolecules with the fluorescent probe acetoacetate 2, 7-dichlorofluorescin cindiacetate (D 399) and Propidium iodide (PI) The level of lipid peroxidation and the degree of cell necrosis in cerebral ischemia and hypoxia injury were observed by optical transection scanning.L NGN arginine (L NNA), N acetylcysteine , NAC) pre-protection can significantly reduce the anoxia and / or reoxygenation injury in rat brain slices, indicating that nitric oxide (NO) and other free radicals play an important role in cerebral anoxia-reoxygenation injury