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目的:研究血管扩张刺激磷蛋白(VASP)与前列腺癌侵袭转移及预后的关系。方法:将VASP shRNA慢病毒和对照shRNA慢病毒分别感染前列腺癌PC3细胞,采用跨膜迁移实验检测PC3细胞的侵袭能力;采用免疫组化法检测56例前列腺癌患者癌组织中VASP的表达,并根据VASP表达差异及患者前列腺癌根治术后随访结果进行生存分析比较。结果:与shRNA慢病毒对照组及空白对照组相比,VASP shRNA慢病毒可抑制前列腺癌PC3细胞VASP的表达,并且显著降低PC3细胞的侵袭能力(P<0.05);对56例前列腺癌患者的生存分析表明,与VASP阴性表达组相比,VASP阳性表达组及VASP强阳性表达组患者生化复发时间显著缩短(P<0.05),后两者之间比较差异无统计学意义(P>0.05)。结论:VASP参与调控前列腺癌PC3细胞的侵袭能力;VASP蛋白表达差异与前列腺癌患者的预后相关。
Objective: To investigate the relationship between Vasodilator stimulated phosphoprotein (VASP) and invasion, metastasis and prognosis of prostate cancer. Methods: PC3 cells were infected with VASP shRNA lentivirus and control shRNA lentivirus respectively. The transmembrane migration assay was used to detect the invasion ability of PC3 cells. The expression of VASP was detected by immunohistochemistry in 56 cases of prostate cancer Survival analysis was performed according to VASP expression differences and follow-up results of patients with radical prostatectomy. Results: Compared with shRNA lentivirus control group and blank control group, VASP shRNA lentivirus could inhibit the expression of VASP in prostate cancer PC3 cells and significantly reduce the invasive ability of PC3 cells (P <0.05). In 56 cases of prostate cancer Survival analysis showed that compared with VASP negative expression group, the biochemical recurrence time of VASP positive expression group and VASP strong positive expression group was significantly shorter (P <0.05), but there was no significant difference between the two groups (P> 0.05) . Conclusion: VASP is involved in the invasion and metastasis of PC3 cells. The difference of VASP expression is related to the prognosis of patients with prostate cancer.