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目的：研究促凋亡基因ｂａｋ在星形细胞瘤（Ａｓｔｒｏｃｙ－ｔｏｍａ）中的表达与意义．方法：采用兔抗人Ｂａｋ多克隆抗体及免疫组织化学ＳＡＢＣ法．结果：在６６例星形细胞瘤中，Ｂａｋ表达３９例（５９．１％）；１０例用于对照的正常脑组织中，Ｂａｋ阳性仅１例（１０％）．ｂａｋ在星形细胞瘤中的表达强于正常对照组（Ｐ＜０．０５），ｂａｋ在Ⅰ，Ⅱ，Ⅲ，Ⅳ级星形细胞瘤相互之间表达无显著差异（Ｐ＞０．０５），但在３１例低度恶性星形细胞瘤（ＷＨＯⅠ级～Ⅱ级）中表达强于在３５例高度恶性星形细胞瘤（ＷＨＯⅢ级～Ⅳ级）中的表达（Ｐ＜０．０５）．结论：促凋亡基因ｂａｋ在大部分星形细胞瘤中有不同程度表达，ｂａｋ可能参与星形细胞瘤的凋亡调变；其表达与星形细胞瘤分化有关．
4. Objective : To study the expression and significance of proapoptotic gene bak in astrocytoma. Methods: Rabbit anti-human Bak polyclonal antibody and immunohistochemistry SABC method were used. RESULTS: In 66 astrocytomas, Bak was expressed in 39 cases (59.1%); in 10 normal brain tissues used for control, only 1 case was Bak positive (10%). The expression of bak in astrocytoma was stronger than that in normal control group (P<0.05), and there was no significant difference in the expression of bak between astrocytoma I, II, III, and IV (P>0.05). However, in 31 cases of low-grade malignant astrocytoma (WHOI grade -II grade), the expression was higher than that in 35 cases of high-grade malignant astrocytoma (WHO III grade -IV grade) (P<0.05). CONCLUSION: The pro-apoptotic gene bak is expressed in different degrees in most astrocytomas, and bak may be involved in the apoptosis modulation of astrocytoma; its expression is related to the differentiation of astrocytoma.