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目的探讨强直性脊柱炎患者外周血中CD4+CD25+Treg(调节性T细胞)、CD4+CD25+CD127 low/-Treg细胞表达水平及其意义,并对两种标识方法检测AS患者Treg进行对比分析。方法采用流式细胞术检测9例正常人和20例强直性脊柱炎患者外周血中的CD4+T细胞比率及CD4+CD25+Treg、CD4+CD25+CD127low/-Treg占CD4+T细胞的比率。结果强直性脊柱炎患者外周血中的CD4+T细胞比率及CD4+CD25+Treg、CD4+CD25+CD127 low/-Treg细胞占CD4+T细胞的比率均明显高于正常对照组(P<0.01)。结论强直性脊柱炎患者免疫功能紊乱,外周血中CD4+CD25+Treg、CD4+CD25+CD127 low/-Treg细胞水平显著增高。两种调节性T细胞标识方法检测强直性脊柱炎患者外周血Treg的结果基本一致。
Objective To investigate the expression and significance of CD4 + CD25 + Treg (Treg), CD4 + CD25 + CD127 low / -Treg in peripheral blood of patients with ankylosing spondylitis and to compare the Tregs detected by two kinds of markers in AS patients analysis. Methods Flow cytometry was used to detect the percentage of CD4 + T cells and the ratio of CD4 + CD25 + Tregs in CD4 + T cells, CD4 + CD25 + CD127low / -Tregs and CD4 + T cells in peripheral blood of 9 normal subjects and 20 patients with ankylosing spondylitis . Results The ratio of CD4 + T cells, CD4 + CD25 + CD127 low / -Tregs and CD4 + T cells in peripheral blood in patients with ankylosing spondylitis were significantly higher than those in normal controls (P <0.01 ). Conclusion The immune dysfunction in patients with ankylosing spondylitis, peripheral blood CD4 + CD25 + Treg, CD4 + CD25 + CD127 low / -Treg cell levels were significantly higher. The results of two regulatory T cell labeling methods for detecting Treg in peripheral blood of patients with ankylosing spondylitis are basically the same.