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目的研究苦皮藤素对白纹伊蚊幼虫的毒杀作用机制及对该蚊幼虫生长发育等各种因素的影响。方法采用常规生物学法进行毒力测定及观察对蚊幼虫生长发育的影响,HE染色观察胃肠形态的改变,通过氧化铬的比色法测定拒食活性。结果随苦皮藤素浓度的增大,其对白纹伊蚊4龄幼虫的毒杀作用力增强,LC50为22.66(18.38～27.95)mg/L,LC95为84.51(60.87～142.27)mg/L。苦皮藤素能降低白纹伊蚊幼虫的化蛹率,延长平均化蛹时间和平均羽化时间,但对羽化率的影响不明显。随苦皮藤素作用浓度的增大,拒食活性也增强。胃肠形态发生改变:刷状缘脱落,中肠细胞变性、崩解。结论苦皮藤素能通过胃毒、拒食、触杀等作用方式毒杀白纹伊蚊幼虫,并能抑制其生长发育。
Objective To study the toxic mechanism of celangulin against Aedes albopictus larvae and its effects on various factors such as the growth and development of the mosquito larvae. Methods The routine biological method was used to determine the virulence and observe the effects on the growth and development of mosquito larvae. The changes of gastrointestinal morphology were observed by HE staining and the antifeedant activity was determined by chromium oxide colorimetry. Results With the increase of cepharanthine concentration, the virulence of cepharanthine against 4th instar larvae of Aedes albopictus was enhanced. The LC50 was 22.66 (18.38 ~ 27.95) mg / L and LC95 was 84.51 (60.87 ~ 142.27) mg / L. Celangulin decreased the pupation rate of Aedes albopictus larvae, prolonged the average pupal time and average emergence time, but had no obvious effect on the emergence rate. As the concentration of cephaladone increased, antifeedant activity also increased. Gastrointestinal changes: brush edge loss, midgut cell degeneration, disintegration. Conclusion Celangulin can poison Aedes albopictus larvae through stomach poisoning, antifeeding, contact killing and other modes of action, and can inhibit its growth and development.