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一、材料与方法 本材料选用猴牙动物实验的部分内容。猴牙移动实验的设计,动物分组,矫治器的制作和正畸力值测定以及取材等请参考作者1988年在实用口腔医学杂志上发表的文章。 样品制备的方法 活体取材的组织块在1%的戍二醛和4%甲醛混合液固定24小时后,用双面金钢砂片沿近远中方向切为两半。每半包括侧切牙的唇侧和舌侧半边齿龈及其周围牙槽骨组织,在砂轮上(点水)磨成1—1.5mm厚的薄骨片,放入2.5%的戊二醛中第二次固定。硫酸和甲酸混合液脱钙2～3天。余按常规H、E染色组织切片的方法处理。
First, the material and methods The material selected monkey teeth animal experiments part. Monkey teeth moving experiment design, animal grouping, appliance production and orthodontic force measurement and drawing, etc. please refer to the author in 1988, published in the Journal of Stomatology articles. Methods of sample preparation Tissue samples from living specimens were fixed in a mixture of 1% glutaraldehyde and 4% formaldehyde for 24 hours and then cut into two halves in both mesiodistal directions using a double-sided diamond plate. Each half of the lateral and lateral gingiva of the lateral incisors and the surrounding alveolar bone tissue, on the wheel (point water) ground into thin slices 1-1.5mm thick, into the glutathione 2.5% Secondary fixation. Mixed sulfuric acid and formic acid decalcification 2 to 3 days. I press conventional H, E staining method of tissue processing.