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试验表明 ,非洲紫罗兰叶片组织培养 ,无论是诱导分化或继代繁殖 ,都以MS +BA 0 1mg/L+NAA 0 1mg/L培养基为好 培养中外植体极性反应明显 ,以不定芽为再生方式 ,可 1次成苗 提高试管苗移栽成活率的关键在于移栽后必须近月的高湿度生长环境 以花叶芋的叶片和叶柄作为离体繁殖的外植体 ,在MS +NAA 0～ 1 0mg/L +KT 0～ 1 0mg/L培养基上培养 ,叶片的增殖效果明显好于叶柄 在最佳配方 (MS +NAA 0 2mg/L +KT 0 2mg/L)培养基上获得的愈伤组织呈颗粒状 ,分散性好 ,胚性细胞多 ,试管苗增殖率在 6倍以上 ,其再生植株率为 10 0 % 另外 ,用生长素NAA和细胞分裂素KT组合配比的MS培养基进行离体快速繁殖可以获得理想的培养效果
The results showed that the polarity reaction of explants of P. violacea in vitro was induced by MS + BA 0 1mg / L + NAA 0 1mg / L both in induced differentiation and subculture, Regeneration method, can be a seedling to improve survival rate of transplanted in vitro transplanting is the key to high-humidity growth environment in recent months to leafy and petiole plantlets as explants in MS + NAA 0-10 mg / L KT 0-1 0 mg / L medium, the proliferation of the leaves was significantly better than that of the petiole on the best medium (MS + NAA 0 2mg / L + KT 0 2mg / L) Of the callus was granular, good dispersibility, more embryogenic cells, the proliferation rate of test tube seedlings more than 6 times, the regeneration plant rate of 10 0% In addition, the combination of auxin NAA and cytokinin KT ratio of MS Medium for rapid propagation in vitro to obtain the desired culture effect