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目的探讨乌鲁木齐市饮用水有机提取物对雄性小鼠的生殖毒性影响。方法选择清洁级雄性ICR小鼠40只(6～8周),分别分为对照(12.5ml/kgDMSO组)、低剂量组(1.25L水中有机提取物/kg组)、中剂量组(2.5L水中有机提取物/kg组)、高剂量组(5L水中有机提取物/kg组)4个剂量组。经口灌胃染毒方式进行染毒,每天1次,连续2周。通过单细胞凝胶电泳实验测定某市饮用水有机提取物对小鼠DNA的影响。结果随着饮用水有机提取物浓度的升高,睾丸彗星试验没有明显差异,小鼠睾丸组织中SOD和MDA的含量与阴性对照组比较差异无统计学意义。结论本实验未发现乌鲁木齐市管网末梢水有机提取物对雄性小鼠氧化性与生殖毒性有明显的影响
Objective To investigate the effects of organic extracts of drinking water in Urumqi on reproductive toxicity of male mice. Methods Forty clean male ICR mice (6-8 weeks) were selected and divided into control group (12.5ml / kg DMSO group), low dose group (1.25L water organic extract / kg group), middle dose group Water organic extract / kg group), high dose group (5L water organic extract / kg group) 4 dose groups. Oral administration of oral administration of poisoning, 1 day, for 2 weeks. The single cell gel electrophoresis assay was used to determine the effects of organic extracts of drinking water in a city on mouse DNA. Results With the increase of concentration of organic extract in drinking water, there was no significant difference in testicular comet assay. The content of SOD and MDA in testis tissue of mice had no significant difference compared with negative control group. Conclusions This experiment did not find that the organic extract of the water at the distal end of the pipe network in Urumqi had a significant effect on the oxidative and reproductive toxicity of male mice