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目的 :探讨 Ki- 6 7抗体对鉴别浆膜腔积液中良恶性细胞的价值 ,解决伴浆膜腔积液的疑难病例的诊断。方法 :用 Ki- 6 7单克隆抗体标记 4 7例浆膜腔积液涂片标本 ,每张涂片计数 10 0 0个细胞中的阳性细胞数 ,用百分率表示阳性指数 ,同时对涂片作 HE染色。结果 :恶性积液组 Ki- 6 7指数为 34.89% ,良性组为 0 .72 % ,两者差异具有显著性 (P<0 .0 0 5 )。结论 :浆膜腔积液涂片 HE染色诊断结合 Ki- 6 7标记 ,可提高恶性浆膜腔积液细胞学的阳性诊断率 ,可作为临床鉴别良恶性浆膜腔积液的参考指标。
Objective: To investigate the value of Ki- 6 7 antibody in differential diagnosis of benign and malignant serous effusions, and to resolve the diagnosis of difficult cases with effusion of serous fluid. Methods: 47 cases of serous cavity effusion specimens were labeled with Ki- 67 monoclonal antibody. The number of positive cells in each of 100 smears was counted, and the positive index was expressed as a percentage. At the same time, HE staining. Results: The Ki- 6 7 index in malignant effusion group was 34.89%, and was 0.72% in benign group. The difference between the two groups was significant (P <0.05). Conclusion: The diagnosis of serosal effusion smear combined with Ki- 6 7 staining can improve the positive diagnosis rate of malignant serous fluid cytology, which can be used as a reference index to differentiate benign and malignant serous effusions.