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目的:探讨HJJB方对脂肪肝大鼠脂质过氧化环节的影响,揭示其防治非酒精性脂肪肝的部分作用机制。方法:采用高脂饮食14周诱导的大鼠非酒精性脂肪肝模型。在造模第9周起,随机分为模型组、HJJB方组和罗格列酮对照组,灌胃给药6周。观察:(1)肝组织病理变化(HE染色);(2)肝组织甘油三酯(TG)、游离脂肪酸(FFA)含量的变化;(3)肝组织丙二醛(MDA)、超氧化物歧化酶(SOD)、一氧化氮合酶(NOS)、诱导型一氧化氮合酶(i NOS)、谷胱甘肽过氧化物酶(GSH-Px)含量的变化;(4)肝组织TG、FFA、MDA、SOD、NOS、i NOS、GSH-Px含量间的相关性分析。结果:模型组肝组织出现显著的肝细胞脂肪变性及空泡样变,肝组织TG、FFA含量较正常组显著升高(P<0.01),肝组织MDA、NOS、i NOS含量较正常组亦明显升高(P<0.01),肝组织GSH-Px、SOD含量较正常组显著降低(P<0.01)。HJJB方组的上述病理改变明显减轻,肝组织TG、FFA、MDA、NOS、i NOS含量较模型组显著降低(P<0.01),肝组织GSH-Px、SOD含量较模型组组显著升高(P<0.05)。肝组织TG、FFA含量与肝组织MDA、NOS、i NOS含量间均呈显著正相关,而与肝组织GSH-Px、SOD含量间则呈显著负相关。结论:HJJB方具有明显的改善脂肪肝大鼠脂质过氧化损伤的作用,这可能是其防治非酒精性脂肪肝的重要机制。
Objective: To investigate the effect of HJJB prescription on lipid peroxidation of fatty liver rats and to reveal its partial mechanism of action in prevention and treatment of non-alcoholic fatty liver disease. Methods: A non-alcoholic fatty liver model induced by a high-fat diet for 14 weeks was used. From the 9th week of modeling, they were randomly divided into model group, HJJB group and rosiglitazone control group. (2) Liver triglyceride (TG), free fatty acid (FFA) content changes; (3) Liver tissue malondialdehyde (MDA), superoxide (SOD), nitric oxide synthase (NOS), inducible nitric oxide synthase (iNOS), glutathione peroxidase (GSH-Px) , FFA, MDA, SOD, NOS, iNOS and GSH-Px levels in patients with acute myocardial infarction. Results: The hepatic steatosis and vacuolar degeneration were observed in the model group. The content of TG and FFA in the liver tissue were significantly higher than those in the normal group (P <0.01), and the contents of MDA, NOS and iNOS in the liver tissue were also significantly higher than those in the normal group (P <0.01). The contents of GSH-Px and SOD in liver tissue were significantly lower than those in normal group (P <0.01). Compared with model group, the contents of TG, FFA, MDA, NOS and iNOS in liver tissue of HJJB group were significantly reduced (P <0.01), and the levels of GSH-Px and SOD in liver tissue were significantly increased P <0.05). There was a significant positive correlation between liver tissue TG, FFA content and liver MDA, NOS, i NOS content, but negative correlation with liver tissue GSH-Px, SOD. Conclusion: HJJB prescription can obviously improve lipid peroxidation injury in fatty liver rats, which may be an important mechanism of prevention and treatment of non-alcoholic fatty liver disease.