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目的 :研究己酮可可碱对血吸虫病肝纤维化小鼠血清一氧化氮及肝内诱导型一氧化氮合酶的影响。方法 :制备血吸虫病肝纤维化小鼠模型 ,应用免疫组化染色方法和多媒体彩色病理图文分析系统 ,定量观察不同剂量己酮可可碱治疗前后肝脏诱导型一氧化氮合酶含量的变化 ,并通过化学还原反应检测血清一氧化氮的水平变化。结果 :应用己酮可可碱治疗后可降低肝内诱导型一氧化氮合酶和血清一氧化氮的含量 ,此作用与治疗剂量有关 ,高剂量治疗组与感染组比较 ,差异有非常显著性 (P <0 .0 1) ;低剂量组与感染组及高剂量组与低剂量组间比较差异亦均具有显著性 (P <0 .0 5 )。结论 :己酮可可碱可显著降低血吸虫病肝纤维化小鼠肝脏诱导型一氧化氮合酶活性及血清一氧化氮含量 ,发挥抗血吸虫病肝纤维化的作用 ,此作用与用药剂量有关。
Objective: To study the effects of pentoxifylline on serum nitric oxide and hepatic inducible nitric oxide synthase in schistosomiasis mice with liver fibrosis. Methods: The model of liver fibrosis in schistosomiasis was prepared. The changes of hepatic inducible nitric oxide synthase (IOS) in different doses of pentoxifylline were observed quantitatively by immunohistochemical staining method and multimedia color chart analysis system Changes in serum nitric oxide levels were measured by chemical reduction. Results: Pentoxifylline decreased the content of inducible nitric oxide synthase and serum nitric oxide in the liver, which was related to the treatment dose. There was a significant difference between the high-dose treatment group and the infected group P <0.01). There was also significant difference between the low-dose group and the infected group and between the high-dose group and the low-dose group (P <0.05). CONCLUSION: Pentoxifylline can significantly reduce hepatic inducible nitric oxide synthase activity and serum nitric oxide synthase in schistosomiasis mice and play an important role in anti-schistosomiasis hepatic fibrosis. This effect is related to the dosage of pentoxifylline.