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目的 通过研究辛伐他汀对高血脂致动脉粥样硬化家兔血管组织蛋白质组的变化 ,评价其药效影响。方法 建立新西兰家兔高脂致动脉粥样硬化模型 ,用双相凝胶电泳技术检测其蛋白质组的变化。结果 服用辛伐他汀后 ,脂肪肝病变基本消失 ;通过分析比较得到的 2DE图像 ,17个点明显上调 (>3倍 ) ,12个点明显下调 (<3倍 ) ,辛伐他汀对其中少数蛋白点有回调作用 ,对多数点无回调作用。结论 辛伐他汀能明显改善肝脏对脂肪的代谢功能 ,但未能有效修复高血脂引起的动脉血管壁的损伤。
Objective To study the effect of simvastatin on proteome in vascular tissue of hypercholesterolemic atherosclerotic rabbits. Methods New Zealand rabbit model of atherosclerosis caused by hyperlipidemia was established, and the changes of proteome were detected by two-phase gel electrophoresis. Results The lesions of fatty liver almost disappeared after taking simvastatin. By analyzing the 2DE images obtained, 17 points were significantly up-regulated (> 3-fold) and 12 points were significantly down-regulated (<3-fold) Point callback effect, no callback on most points. Conclusion Simvastatin can significantly improve the metabolic function of liver fat, but failed to effectively repair hyperlipidemia caused by arterial wall damage.