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建立了测定皮蛋中Hg的双波长共振瑞利散射新方法,研究了共振瑞利散射的光谱特征、共存物质的影响及反应机理。在pH 6.58的Tris-HCl缓冲介质中,Hg(Ⅱ)与快绿-溴代十六烷基吡啶结合生成三元络合物,使体系的双波长-共振瑞利散射(DWO-RRS)光谱显著增强,最大和次大的共振瑞利散射峰分别位于344 nm和470 nm波长处。当用双波长-共振瑞利散射(DWO-RRS)法测定时,Hg(Ⅱ)的质量浓度在0.005~0.50 mg/L范围内与体系的共振瑞利散射增强强度△I_(RRS)呈线性关系,检出限(3σ/S)为1.4 ng/mL,定量限为0.034 mg/kg。方法用于皮蛋中Hg的测定,回收率为98.2%~102%,相对标准偏差(n=5)为2.1%~2.7%。
A new method of dual wavelength resonance Rayleigh scattering for the determination of Hg in preserved eggs was established. The spectral characteristics of resonance Rayleigh scattering, the influence of coexisting substances and the reaction mechanism were studied. In Tris-HCl buffering buffer at pH 6.58, Hg (Ⅱ) combined with fast green-bromodecyl pyridinium to form ternary complex, which made the double-wavelength-resonance Rayleigh scattering (DWO-RRS) The maximum and maximum resonance Rayleigh scattering peaks are at 344 nm and 470 nm, respectively. The mass concentration of Hg (Ⅱ) was linear with the enhanced RRS of the system in the range of 0.005-0.50 mg / L when measured by DWO-RRS (double wavelength-resonance Rayleigh scattering) The detection limit (3σ / S) was 1.4 ng / mL and the limit of quantification was 0.034 mg / kg. The method was applied to the determination of Hg in preserved eggs with a recovery of 98.2% -102% and a relative standard deviation (n = 5) of 2.1% -2.7%.