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目的:探讨重组β2糖蛋白Ⅰ(rβ2GPⅠ)在弱精子症治疗中的应用价值。方法:将100例弱精子症患者的精液标本每份分为4份,1份为正常生理盐水对照组,其余3份分别与5μmol/L(低剂量组)、10μmol/L(中剂量组)和20μmol/L(高剂量组)的rβ2GPⅠ于37℃孵育2 h,计算机辅助精液分析系统(CASA)进行精液常规分析。结果:低、中、高剂量组a+b级精子百分率均显著高于对照组(P均<0.05);中、低剂量组间a+b级精子百分率差异无统计学意义(P>0.05);高剂量组的a+b级精子百分率显著高于中、低剂量组(P<0.05);中、高剂量组的精子平均直线运动速度(VSL)、平均侧摆幅值(ALH)、运动的直线性(LIN)、运动的摆动性(WOB)和运动的前向性(STR)均显著高于对照组(P<0.05);高剂量组的精子VSL、ALH、LIN、WOB和STR显著高于中、低剂量组(P<0.05)。结论:rβ2GPⅠ能够以浓度依赖的方式提高体外精子的活力,为临床弱精子症的治疗提供了新的思路和方法。
Objective: To investigate the value of recombinant β2 glycoprotein Ⅰ (rβ2GPⅠ) in the treatment of asthenospermia. Methods: Semen samples from 100 patients with asthenospermia were divided into 4 parts, 1 part normal saline control group and the other 3 parts were compared with 5μmol / L (low dose), 10μmol / L (medium dose) And 20μmol / L (high dose group) of rβ2GP Ⅰ incubated at 37 ℃ for 2 h, the computer-assisted semen analysis system (CASA) for routine analysis of semen. Results: The percentage of a + b sperm in low, medium and high dose groups was significantly higher than that in control group (all P <0.05). There was no significant difference in the percentage of a + b sperm between middle and low dose groups (P> 0.05) ; The sperm percentage of a + b in the high dose group was significantly higher than that in the medium and low dose groups (P <0.05); the sperm mean linear velocity (VSL), mean lateral swing (ALH) LIN, WOB and STR were significantly higher than those in control group (P <0.05). The sperm VSL, ALH, LIN, WOB and STR in high dose group were significantly higher than those in control group Higher than medium and low dose group (P <0.05). CONCLUSION: rβ2GPⅠ can increase the sperm motility in vitro in a concentration-dependent manner, providing a new way of thinking for the treatment of clinical asthenospermia.