论文部分内容阅读
背景与目的:间变性淋巴瘤激酶(ALK)是系统性间变性大细胞淋巴瘤(ALCL)较为特异的标志,近来研究发现一种新的蛋白clusterin在ALCL中也有较高的表达率,其可能在ALCL的发展中起作用,并对诊断和治疗具有潜在价值。本研究探讨ALCL中ALK及clusterin蛋白表达特点、相互关系及临床病理意义。方法:应用免疫组织化学EnVision法检测90例淋巴瘤组织中ALK及clusterin蛋白表达,其中包括47例ALCL及对照组周围T细胞淋巴瘤非特殊型(PTCL-u)22例,经典型霍奇金淋巴瘤(CHL)21例。结果:ALK在ALCL、PTCL和CHL中的阳性率分别为60%(28/47)、0和0;clusterin在三者中的阳性率分别81%(38/47)、27%(6/22)和14%(3/21),ALK和clusterin在ALCL的阳性率均显著高于PTCL和CHL的阳性率(P<0.05),clusterin在ALK阴性的ALCL中的阳性率为68%(13/19),也显著高于PTCL和CHL的阳性率(P<0.05)。ALK阳性的ALCL中位年龄20岁(3~70岁),显著低于ALK阴性者(P<0.05),阴性中位年龄48岁(4~71岁),ALK阳性与否与发生部位、性别无关(P>0.05)。clusterin的表达与否与年龄、部位和性别均无关(P>0.05)。结论:ALK在ALCL中的特异性表达对其诊断、鉴别诊断并可能对临床预后判断具有重要价值。clusterin作为一新的分子标志物,在ALCL中的相对特异性高表达对ALCL的诊断、特别是对ALK阴性的ALCL与PTCL和CHL的鉴别诊断将具有重要意义。
BACKGROUND & AIM: Atypical Anaplastic Lymphoma Kinase (ALK) is a more specific marker of systemic anaplastic large cell lymphoma (ALCL). Recently, a new protein, clusterin, has also been found to have a higher expression rate in ALCL. Play a role in the development of ALCL and have potential value for diagnosis and treatment. This study was to investigate the expression, correlation and clinicopathological significance of ALK and clusterin proteins in ALCL. Methods: The expressions of ALK and clusterin in 90 cases of lymphoma were detected by EnVision immunohistochemistry, including 47 cases of ALCL and 22 cases of T-cell lymphoma non-special type (PTCL-u) around the control group. The classical Hodgkin’s Lymphoma (CHL) in 21 cases. Results: The positive rates of ALK in ALCL, PTCL and CHL were 60% (28/47), 0 and 0 respectively. The positive rates of clusterin were 81% (38/47), 27% (6/22) ) And 14% (3/21) respectively. The positive rates of ALK and clusterin in ALCL were significantly higher than those in PTCL and CHL (P <0.05). The positive rate of clusterin in ALK negative ALCL was 68% (13 / 19), also significantly higher than the positive rate of PTCL and CHL (P <0.05). The median age of ALK-positive ALCL was 20 years old (range 3 to 70 years), significantly lower than that of ALK negative (P <0.05), and the median age was 48 years (range 4-71 years) Not related (P> 0.05). The expression of clusterin was not related to age, location and gender (P> 0.05). CONCLUSIONS: The specific expression of ALK in ALCL is of great value in the diagnosis and differential diagnosis of ALK, and may be useful in judging clinical prognosis. Clusterin as a new molecular marker, the relative specificity of ALCL in high expression of ALCL diagnosis, especially for ALK-negative ALCL and PTCL and CHL differential diagnosis will be of great significance.