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本文对大麦品种Ingrid(Mlo)及Mlo基因功能丧失性突变的近等基因系(Ingridmlo-3、Ingridmlo-4和Ingridmlo-5)与大麦叶枯病菌的互作进行了细胞学比较分析。结果发现,大麦mlo突变体mlo-3、mlo-4和mlo-5对叶枯病菌的抗病性显著增强,其抗病机制包括对病菌初侵染的乳突抗性和HR反应两个方面。Ingrid(Mlo)大麦的表皮细胞在受到病原菌侵染时,只有15%的有效乳突可成功抵抗病原菌对表皮细胞的侵染;而大麦mlo突变体mlo-3、mlo-4和mlo-5突变体大麦的有效乳突率则分别高达40%、38%和43%。随后,部分被病原菌成功侵入的寄主表皮细胞发生过敏性坏死,从而进一步抑制病原菌向相邻叶肉细胞的扩展。其中3个大麦mlo基因突变体平均67%的被侵染细胞坏死,而Ingrid仅为26%。因此,Mlo基因在调控大麦与不同病原菌互作中的作用是不同的。此外,本文发现该病原菌除气孔入侵外,还可直接穿透大麦表皮细胞;少量菌丝可以侵染进入叶肉细胞,甚至是活的叶肉细胞。
In this paper, cytological analysis of interactions between the proximal germplasm lines (Ingridmlo-3, Ingridmlo-4 and Ingridmlo-5) of the barley variety Ingrid (Mlo) and Mlo gene loss- The results showed that the resistance of mlo-3, mlo-4 and mlo-5 mlo-3 and mlo-5 to barley leaf blight were significantly increased, and their resistance mechanisms included two aspects: the resistance to papillae and the HR reaction . Epidermal cells of Ingrid (Mlo) barley successfully infected only 15% of the effective papilla cells infected with pathogens when infected by pathogenic bacteria. However, mutations in mlo-3, mlo-4 and mlo-5 of barley mlo mutants Effective barley rates for body barley are as high as 40%, 38% and 43%, respectively. Subsequently, some of the host epidermal cells that have been successfully invaded by pathogens undergo allergic necrosis, further inhibiting the expansion of pathogens to adjacent mesophyll cells. An average of 67% of the infected cells in three mow mutant lines of barley were necrotic, while Ingrid was only 26%. Therefore, the role of Mlo gene in the regulation of barley interactions with different pathogens is different. In addition, we found that the pathogen could directly penetrate barley epidermal cells in addition to stomatal invasion. A small amount of mycelium could infect mesophyll cells and even mesophyll cells.