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[目的]探讨趋化因子CXCL12对人胃癌SGC-7901细胞增殖及趋化因子受体CXCR4,CXCR7和血管内皮生长因子(VEGF)、基质金属蛋白(MMP)-2,MMP-9mRNA表达的影响.[方法]取体外培养的人胃癌SGC-7901细胞,用不同质量浓度的CXCL12(0.1,1.0,10.0,100.0μg/L)处理后培养72 h,采用MTT法检测人胃癌SGC-7901细胞的增殖率;用CXCL12(10.0μg/L)处理人胃癌SGC-7901细胞48h后,应用RT-PCR检测细胞内CXCR4,CXCR7,VEGF,MMP-2及MMP-9 mRNA的表达水平.[结果]不同质量浓度的CXCL12均可明显促进人胃癌SGC-7901细胞的增殖率(P<0.001),CXCL12可显著上调CXCR4及CXCR7 mRNA的表达(P<0.001,P<0.01),增加VEGF,MMP-2及MMP-9 mRNA的表达(P<0.05).[结论]趋化因子CXCL12可促进人胃癌SGC-7901细胞的增殖,其机制可能与上调CXCR4及CXCR7表达,从而增加VEGF,MMP-2及MMP-9表达有关.
[Objective] To investigate the effect of chemokine CXCL12 on the proliferation of human gastric cancer cell line SGC-7901 and the expression of CXCR4, CXCR7, VEGF, MMP-2 and MMP-9 mRNA. [Methods] Human gastric cancer SGC-7901 cells cultured in vitro were cultured in different concentrations of CXCL12 (0.1, 1.0, 10.0 and 100.0 μg / L) for 72 h. The proliferation of SGC-7901 cells was detected by MTT assay The expression of CXCR4, CXCR7, VEGF, MMP-2 and MMP-9 mRNA in human gastric cancer SGC-7901 cells treated with CXCL12 (10.0 μg / L) for 48 h were detected by RT-PCR. [Results] (P <0.001). CXCL12 could up-regulate the expression of CXCR4 and CXCR7 mRNA (P <0.001, P <0.01), and increase the expression of VEGF, MMP-2 and MMP in human gastric cancer cell line SGC-7901 (P <0.05). [Conclusion] The chemokine CXCL12 can promote the proliferation of human gastric cancer cell line SGC-7901. The mechanism may be related to up-regulating the expression of CXCR4 and CXCR7 and increasing the expression of VEGF, MMP-2 and MMP- Expression related.