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目的探讨子宫颈炎组织和子宫颈癌组织中GRP78、CRT、ERP57蛋白与HPV16型感染在维吾尔族和汉族妇女中的表达及相关关系。方法采用免疫组化SP法和PCR技术检测子宫颈炎组织69例(汉族38例,维吾尔族31例)以及子宫颈癌组织124例(汉族36例,维吾尔族88例)中GRP78、CRT、ERP57蛋白的表达和HPV16的感染情况。结果 HPV16型感染在子宫颈癌中的阳性表达率(31.45%)高于宫颈炎组(11.59%),差异有统计学意义(P<0.05)。GRP78、CRT、ERP57 3种蛋白在宫颈癌中的表达(95.16%、90.32%、90.32%)均高于与其各自在宫颈炎中的表达(89.96%、50.72%、49.28%),差异均有统计学意义(P<0.05)。GRP78和CRT在汉族妇女宫颈癌中的表达(100.00%、97.22%)均高于各自在汉族妇女宫颈炎中的表达(81.58%、39.47%),并且差异均有统计学意义(P<0.05)。CRT和ERP57在维吾尔族妇女宫颈癌中的表达(87.50%、94.32%)均高于各自在维吾尔族妇女宫颈炎中的表达(64.51%、35.48%),并且差异均有统计学意义(P<0.05)。除此之外,仅ERP57在维吾尔族妇女宫颈癌中的表达(94.32%)高于其在汉族宫颈癌中的表达(80.56%),差异有统计学意义(P<0.05)。而通过对GRP78、CRT、ERP57蛋白与HPV16的相关性分析发现:GRP78、CRT、ERP57蛋白在维吾尔族妇女宫颈癌中的阳性表达率与HPV16型感染均存在正相关关系(r=0.278,P<0.05;r=0.429,P<0.05;r=0.222,P<0.05)。结论通过对GRP78、CRT、ERP57 3种蛋白和HPV16联合检测,可能作为检查子宫颈癌的标记物,提高宫颈癌的检出率;ERP57蛋白在宫颈癌中的表达存在民族间差异。
Objective To investigate the expression and correlation of GRP78, CRT, ERP57 protein and HPV16 infection in Uighur and Han women with cervicitis and cervical cancer. Methods Immunohistochemical SP method and PCR were used to detect the expression of GRP78, CRT, ERP57 in 69 cases of cervicitis (38 cases of Han and 31 cases of Uygur) and 124 cases of cervical cancer (36 cases of Han and 88 cases of Uygur) Protein expression and HPV16 infection. Results The positive rate of HPV16 infection in cervical cancer (31.45%) was higher than that in cervicitis (11.59%), the difference was statistically significant (P <0.05). The expression of GRP78, CRT and ERP57 in cervical cancer (95.16%, 90.32%, 90.32%) were higher than those in cervicitis (89.96%, 50.72%, 49.28%, respectively) Significance (P <0.05). The expression of GRP78 and CRT in Han women with cervical cancer (100.00%, 97.22%) were higher than that of Han women with cervicitis (81.58%, 39.47%, respectively), and the differences were statistically significant (P0.05) . The expression of CRT and ERP57 in Uighur women with cervical cancer (87.50%, 94.32%) were higher than that in Uyghur women with cervicitis (64.51%, 35.48%, respectively), and the differences were statistically significant (P < 0.05). In addition, the expression of ERP57 only in Uighur women with cervical cancer (94.32%) was higher than that in Han women (80.56%), the difference was statistically significant (P <0.05). The correlation analysis of GRP78, CRT, ERP57 protein and HPV16 showed that the positive expression rates of GRP78, CRT and ERP57 protein in Uigur women cervical cancer were positively correlated with HPV16 infection (r = 0.278, P < 0.05; r = 0.429, P <0.05; r = 0.222, P <0.05). Conclusion The combined detection of GRP78, CRT, ERP57 and HPV16 may be used as a marker of cervical cancer to improve the detection rate of cervical cancer. The expression of ERP57 protein in cervical cancer may have inter-ethnic differences.