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目的探讨Seprase在上皮性卵巢癌胸腹水癌细胞中的表达及其临床预后意义。方法选择郑州大学第一附属医院和挪威奥斯陆大学附属肿瘤医院2001~2003年住院的65例上皮性卵巢癌患者的胸腹水标本75份,采用免疫组化技术检测了65份卵巢癌腹水和10份胸水细胞石蜡包埋组织中Seprase蛋白的表达,并对其中34份新鲜腹水细胞进行免疫印迹分析,同时检测9份良性腹水脱落细胞作为对照。结果上皮性卵巢癌胸腹水中Seprase蛋白阳性表达率77.33%(58/75),显著高于良性胸腹水的22.22%(2/9),P<0.01。Seprase蛋白的表达与卵巢癌患者年龄、FIGO分期、组织学分级和组织学类型无关,而与患者预后呈负相关(P<0.05)。蛋白印迹表明27/34样本中同时检测到170kuSeprase二聚体和97ku单体,1份仅表达170kuSeprase二聚体。结论Se-prase参与了卵巢癌的发生发展,并与患者不良预后有关,可能成为卵巢癌的预后指标。
Objective To investigate the expression of Seprase in human ascites carcinoma cells and its prognostic significance. Methods Seventy-five samples of pleural effusion and ascites were collected from 65 patients with epithelial ovarian cancer who were admitted to the First Affiliated Hospital of Zhengzhou University and Cancer Hospital of Oslo University in Norway from 2001 to 2003. Immunohistochemistry was used to detect 65 ascites in ovarian cancer and 10 The expression of Seprase protein in paraffin-embedded tissues of pleural effusion cells was evaluated by immunoblotting in 34 fresh ascites cells. Nine benign ascites exfoliated cells were also detected as controls. Results The positive rate of Seprase protein in pleural effusion and ascites of epithelial ovarian cancer was 77.33% (58/75), which was significantly higher than that of benign pleural effusion (22.22%, 2/9), P <0.01. The expression of Seprase protein was not related to the age, FIGO stage, histological grade and histological type of ovarian cancer patients, but negatively correlated with the prognosis (P <0.05). Western blotting showed that both 170 kuSeprase dimer and 97 ku monomer were detected in 27/34 samples and 1 ku was only expressing 170 kuSeprase dimer. Conclusion Se-prase is involved in the occurrence and development of ovarian cancer, which is related to the poor prognosis of patients and may be the prognostic indicator of ovarian cancer.