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目的了解人类免疫缺陷病毒I型(HIV-1)B′亚型特异性细胞毒性T细胞(CTL)功能与中国HIV-1感染者疾病进展关系。方法将覆盖HIV-1B′亚型Gag p17、p24和p2p7plp6全长的54个重叠多肽作为抗原,用酶联免疫斑点实验(ELISPOT)检测58例HIV-1感染者特异性CTL对上述多肽的应答情况。结果中国HIV-1感染者特异性CTL可识别多个HIV-1B′Gag表位,反应宽度与病毒载量显著负相关(r=-0.374,P=0.004),与CD4+T细胞绝对计数显著正相关(r=0.425,P=0.001),反应强度与病毒载量显著负相关(r=-0.285,P=0.030)。长期不进展者识别HIV-1B′Gag多肽的反应宽度显著高于无症状感染者和艾滋病患者(P=0.001,P=0.005)。结论我国HIV-1感染者体内存在识别不同HIV-1B′Gag多肽表位的特异性CTL应答,并且与疾病进展相关。
Objective To understand the relationship between the immunosuppression of human immunodeficiency virus type 1 (HIV-1) B ’subtype-specific cytotoxic T lymphocyte (CTL) and the progression of HIV-1 infection in China. Methods Fifty-four HIV-1 isoforms, Gag p17, p24 and p2p7plp6, were used as antigens to detect the response of 58 HIV-1-infected CTLs to the above peptides by ELISPOT Happening. Results The specific CTLs of HIV-1 infected in China were able to recognize multiple HIV-1B’Gag epitopes. The response width was significantly and negatively correlated with viral load (r = -0.374, P = 0.004), with significant absolute counts of CD4 + T cells (R = 0.425, P = 0.001). There was a significant negative correlation between the reaction intensity and viral load (r = -0.285, P = 0.030). The response length of HIV-1B’Gag peptides recognized by long-term non-progressors was significantly higher than that of asymptomatic and AIDS patients (P = 0.001, P = 0.005). Conclusions There is a specific CTL response in HIV-1 infected individuals in our country that recognizes different HIV-1B’Gag polypeptide epitopes and is associated with disease progression.