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目的研究低浓度姜黄素(curcumin)对脂多糖(lipopolysaccharide,LPS)活化巨噬细胞产生活性氧(reactive oxygen species,ROS)和发生凋亡的影响。方法体外培养小鼠巨噬细胞RAW264.7,用LPS(0、0.5、2和8μg/m L)诱导细胞,或采用低浓度姜黄素(7.5μmol/L)与LPS同时作用于巨噬细胞;流式细胞术分析活性氧(ROS)、线粒体膜电位(mitochondrial membrane potential,MMP)和细胞凋亡。结果随LPS浓度增加,小鼠巨噬细胞内ROS增加;高浓度的LPS导致细胞线粒体功能损伤和凋亡;与对照组比较,姜黄素处理后,细胞内活性氧由(8.1±0.8)下降到(6.6±1.3),差异有统计学意义(P<0.05);与LPS单独作用比较,姜黄素处理后细胞内活性氧由(61.9±5.3)下降到(47.9±3.8),差异有统计学意义(P<0.05);低浓度的姜黄素对RAW264.7细胞MMP无明显影响,但与LPS单独作用比较,姜黄素处理后细胞内MMP由(37.9±4.7)下降到(33.1±3.4),差异有统计学意义(P<0.05);低浓度姜黄素单独作用未导致发生明显凋亡或坏死,与LPS作用比较,姜黄素明显减少LPS活化后凋亡和坏死细胞的百分率,差异有统计学意义(P<0.05)。结论低浓度姜黄素降低了ROS而减轻LPS活化小鼠巨噬细胞的凋亡。
Objective To investigate the effects of curcumin at low concentration on the production of reactive oxygen species (ROS) and apoptosis in macrophages activated by lipopolysaccharide (LPS). Methods Macrophage RAW264.7 cells were cultured in vitro, and induced by LPS (0, 0.5, 2 and 8 μg / mL), or treated with LPS at a low concentration (7.5 μmol / L) Flow cytometry analysis of reactive oxygen species (ROS), mitochondrial membrane potential (MMP) and apoptosis. Results ROS increased in macrophages with the increase of LPS concentration. High concentrations of LPS resulted in mitochondrial dysfunction and apoptosis. Compared with the control group, the intracellular ROS decreased from (8.1 ± 0.8) to (6.6 ± 1.3), the difference was statistically significant (P <0.05). Compared with LPS alone, the intracellular reactive oxygen species decreased from (61.9 ± 5.3) to (47.9 ± 3.8) after curcumin treatment, the difference was statistically significant (P <0.05). However, curcumin at a low concentration had no significant effect on MMPs in RAW264.7 cells, but compared with LPS alone, the intracellular MMP decreased from (37.9 ± 4.7) to (33.1 ± 3.4) after curcumin treatment, (P <0.05); curcumin at a low concentration alone did not lead to significant apoptosis or necrosis. Compared with LPS, curcumin significantly decreased the percentage of apoptotic and necrotic cells after LPS activation, the difference was statistically significant (P <0.05). Conclusion Low concentration of curcumin reduces ROS and decreases the apoptosis of LPS-activated macrophages in mice.