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目的:确定l-SPD在基底神经核的作用部位。方法:6-OHDA损毁大鼠单侧黑质致密区(SNC),核团内微注或微电泳给予l-SPD或DA激动剂,作旋转实验和神经元放电记录。结果:1)大鼠纹状体损毁侧DA免疫反应物减少。2)新纹状体或黑质网质区(SNR)内微注DA激动剂Apo(D_1/D_2),SK＆F38393(D_1)或SPD引起大鼠强烈旋转,而微注Ly171555(D_2)于SNR或DA激动剂和l-SPD于苍白球(GP)均不引起旋转。卡英酸进一步损毁GP或脚间核(EP),DA激动剂或l-SPD诱发大鼠旋转显著下降。3)微电泳Apo或SPD引起SNR神经元放电,但对GP神经元无效。结论:l-SPD诱发大鼠旋转和神经元放电由D_1受体介导,基底核新纹状体和SNR是其作用部位,而不是GP。通过SNR的直接环路在旋转行为中起重要作用。
Objective: To determine the role of l-SPD in the basal ganglia. Methods: Unilateral substantia nigra dense zone (SNC) of 6-OHDA-lesioned rats were treated with l-SPD or DA agonist by intra-nucleus microinjection or micro-electrophoresis for spin test and neuronal discharge recording. Results: 1) Decreased DA immune response in rat striatum. 2) ATR (D_1 / D_2), SK & F38393 (D_1), or SPD induced by microinjection of DA agonist in the new striatum or the substantia nigra reticulum (SNR) resulted in a strong rotation in rats, whereas microinjection of Ly171555 (D_2) DA agonists and l-SPD did not cause rotation in the globus pallidus (GP). Cardiac acid further damaged the GP or interventricular nucleus (EP), DA agonists or l-SPD-induced rat spin significantly decreased. 3) Microelectrophoresis Apo or SPD causes SNR neuronal discharge, but not GP neurons. CONCLUSIONS: l-SPD-induced rat spin and neuronal firing are mediated by D-1 receptors. The striatum and SNR of basal ganglia are the site of action rather than GP. The direct loop through the SNR plays an important role in the rotation behavior.