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本文报道应用常规细胞内微电极技术记录原代单层培养大鼠搏动心肌细胞感染 Coxsackie B-2病毒后不同时间的电生理参数变化和同时期在倒置相差显微镜下观察的心肌细胞搏动频率、节律、搏动%及细胞病变。在此基础上,研究黄芪对 Coxsackie B-2病毒感染体外培养搏动大鼠心肌细胞电活动的影响。结果提示从病毒感染后24～96h,黄芪对心肌细胞搏动频率、节律、搏动%、细胞病变和各项电生理参数等均有明显保护作用,心肌细胞的结构和功能均维持在大致正常状态。本文结果可为黄芪防治病毒性心肌炎提供新的线索。
This article reports the use of conventional intracellular microelectrode technique to record the changes of electrophysiological parameters at different time after the infection of Coxsackie B-2 virus in primary monolayer cultured rat beating cardiomyocytes and the beat frequency and rhythm of myocardial cells observed under inverted phase contrast microscope at the same time. , beats% and cell lesions. On this basis, the effects of Astragalus membranaceus on Coxsackie B-2 virus-infected rat myocardial cells in vitro were studied. The results suggest that from 24 to 96 hours after viral infection, Astragalus membranaceus has obvious protective effects on myocardial beat frequency, rhythm, pulsation%, cytopathological changes and various electrophysiological parameters, and the structure and function of cardiac myocytes are maintained at approximately normal state. This article can provide new clues for the prevention and treatment of viral myocarditis by Astragalus membranaceus.