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病毒检测在预防和控制对虾病毒性疫病实践中具有重要的作用和意义。为了提高对虾病毒检测的快速性和简便性,建立了一种可同时检测6种对虾病毒的RT-PCR方法,并利用该方法对来自广西沿海地区的130份病虾样品中的黄头病毒(YHV)、白斑综合征病毒(W SSV)、桃拉病毒(TSV)、肝胰腺坏死病毒(HPV)、传染性皮下组织坏死病毒(IHHNV)以及杆状病毒(MBV)进行了检测。检测结果:白斑综合征病毒发病率最高,检出率高达73.8%;传染性皮下组织坏死病毒发病率为18.5%;桃拉病毒检出率为3.1%;未检出黄头病毒、肝胰腺坏死病毒和杆状病毒。该RT-PCR方法扩增得到的片段经测序,结果与标准毒株的核酸数据高度吻合。实践证明,该实验建立的RT-PCR方法具有高度的准确性,与其他检测方法相比具有快速、简便的特点,在对虾病毒检疫、流行病学调查等方面具有重要的应用价值。
Viral detection plays an important role in the prevention and control of shrimp viral diseases. In order to improve the rapidity and simplicity of detection of shrimp virus, an RT-PCR method was developed to detect six shrimp viruses at the same time. Based on the RT-PCR method, sixty shrimp viruses from 130 shrimp samples in the coastal area of Guangxi YHV, W SSV, TSV, HPV, IHHNV and baculovirus (MBV) were detected. The results showed that the incidence of white spot syndrome virus was the highest, the detection rate was as high as 73.8%; the incidence of infectious subcutaneous tissue necrosis virus was 18.5%; the detection rate of taura virus was 3.1%; yellow virus, hepatic and pancreatic necrosis Viruses and baculoviruses. The fragment amplified by this RT-PCR method was sequenced and the results were in good agreement with the nucleic acid data of the standard strains. Practice has proved that the RT-PCR method established by the experiment has a high degree of accuracy, compared with other detection methods has the characteristics of quick and easy, in shrimp virus quarantine, epidemiological investigation has important application value.