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目的 对四川省 2 0 0 1- 2 0 0 2年流感病原学及血清学监测结果进行分析。方法 用狗肾传代细胞 (MDCK)和鸡胚双腔法进行流感病毒分离 ;用血凝抑制实验对攀枝花、雅安和德阳等 3个流感监测点的 14 3名健康人血清进行流感病毒抗体检测。结果 2年共采集流感样患者标本 1838份 ,分离出流感病毒 78株 ,其中甲1、甲3和乙型流感病毒分别为 4 0株、 3株和 35株。四川省健康人群对A/上海 / 7/ 99(H1N1)、A/福建 / 15 1/ 2 0 0 0 (H3N2 )、B/上海 / 2 0 / 2 0 0 1和B/浙江 / 2 / 2 0 0 1流感病毒的血清抗体阳性率分别是 96 5 %、 10 0 0 %、 96 5 %和 6 7 8%。结论 2 0 0 1- 2 0 0 2年四川省流感的流行情况较为平静 ,流行优势株分别为乙型和甲1亚型流感病毒。
Objective To analyze the results of influenza aetiology and serological surveillance in Sichuan from 2000 to 2002. Methods Influenza virus was isolated from dog’s passaged cells (MDCK) and chicken embryo double cavity method. The serum of 14 3 healthy volunteers in three flu surveillance stations of Panzhihua, Ya’an and Deyang was detected by hemagglutination inhibition test. Results A total of 1838 influenza virus samples were collected in 2 years and 78 strains of influenza virus were isolated. Among them, 40 strains of A, 3 A and B influenza viruses were 4, 3 and 35, respectively. Sichuan Province, the healthy population of A / Shanghai / 7/99 (H1N1), A / Fujian / 15 1/2000 (H3N2), B / Shanghai / 20/2001 and B / Zhejiang / 2/2 The positive rates of serum antibodies of influenza virus were 96 5%, 100%, 96 5% and 67 8% respectively. Conclusions The prevalence of influenza in Sichuan Province in 2000-2002 was relatively calm. The predominant strains were influenza A and B subtype A viruses.