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目的探讨环磷酰胺(CTX)诱导膀胱肿瘤的机制。方法 SD大鼠12只,随机分为两组,处理组和对照组各6只。处理组大鼠给与环磷酰胺100mg/kg腹腔注射,对照组给与同等剂量的生理盐水腹腔注射;24h后取膀胱,采用HE染色观察形态结构的变化,采用免疫组织化学方法检测KI67、P53、P21的表达定位,采用Western blot的方法检测KI67、P53、P21的蛋白表达量,并进行数据统计。结果 CTX处理组膀胱出现明显的水肿、出血、粘连;HE染色可见,膀胱黏膜弯曲减少、肿胀,细胞排列疏松,膀胱黏膜呈现毛刺样改变;免疫组织化学染色可见KI67/P53/P21在膀胱黏膜上皮细胞表达;Western blot检测及数据统计可知,KI67蛋白在CTX组表达高于对照组(P<0.05);P53/P21蛋白表达在CTX组低于对照组(P<0.05)。结论环磷酰胺可降低膀胱黏膜P53/P21蛋白的表达,诱导膀胱肿瘤的发生。
Objective To investigate the mechanism of cyclophosphamide (CTX) -induced bladder cancer. Methods Twelve SD rats were randomly divided into two groups, six in each group. The rats in the treatment group were given intraperitoneal injection of cyclophosphamide (100mg / kg), and the control group were injected intraperitoneally with the same dose of saline. After 24h, the bladder was taken and the morphological changes were observed by HE staining. Immunohistochemistry was used to detect the expression of KI67, P53 , P21 expression and localization. Western blot was used to detect the protein expression of KI67, P53 and P21, and the data were statistically analyzed. Results The cyst of CTX treated group showed obvious edema, hemorrhage and adhesion. HE staining showed that the mucosa of bladder showed a decrease in curvature, swelling and loosely arranged cells, and the mucosa of the bladder showed burr-like changes. Immunohistochemical staining showed that KI67 / P53 / The expression of KI67 protein in CTX group was higher than that in control group (P <0.05). The expression of P53 / P21 protein in CTX group was lower than that in control group (P <0.05). Conclusion Cyclophosphamide can reduce the expression of P53 / P21 protein in bladder mucosa and induce the occurrence of bladder cancer.