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目的建立黄连炉甘石洗剂盐酸小檗碱含量的质量控制标准。方法采用高效液相色谱法测定处方中盐酸小檗碱的含量。结果建立高效液相色谱法测定处方中的盐酸小檗碱含量的方法。盐酸小檗碱的进样量在10~100μg/ml范围内呈良好的线性关系(R2=0.9996),平均回收率为102.01%,RSD为0.49%(n=6)。结论本方法专属性强、准确、重现性好,可用于黄连炉甘石洗剂盐酸小檗碱含量的质量控制标准。
Objective To establish the quality control standard of berberine hydrochloride in Huanglian calamine lotion. Methods The content of berberine hydrochloride in prescription was determined by high performance liquid chromatography. Results Establishment of HPLC method for the determination of the content of berberine hydrochloride. Berberine hydrochloride showed a good linearity (R2 = 0.9996) with an average recovery of 102.01% and a RSD of 0.49% (n = 6) in the range of 10-100 μg / ml. Conclusion The method is specific, accurate and reproducible. It can be used for quality control of berberine hydrochloride in the berberine calamine lotion.