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(4)抗药性测定一般应考虑的问题首无要确定已知敏感品系的基线数据,包括LC_(50),LC_(99.9)以及回归式的坡度(b)和异质系数(heterogeneity factors,即HF)等等.这些数据要求在统一的实验条件下,一般温度为25℃,相对湿度为70%,利用昆虫统一的年龄(2-4周)的成虫,在特定的处理时间测定剂量死亡率的直线方程,实验要求最少采用4个等级浓度和3个重复.然后利用诊断剂量(diagnostic dose)对抗性样品进行初选,初选试验诊断
(4) General considerations for drug resistance determination First, baseline data of known sensitive strains should be identified, including LC_ (50), LC_ (99.9), regression slope (b) and heterogeneity factors HF), etc. These data require that adult adults of uniform insect age (2-4 weeks) be tested for their dose-specific mortality at specific treatment times under uniform experimental conditions, typically at 25 ° C and a relative humidity of 70% Of the linear equation, the experiment requires a minimum of 4 levels of concentration and 3 repeats.Then the use of diagnostic dose (diagnostic dose) against primaries primary sample, primary diagnostic test