为证实喉癌的发生发展与抗癌基因Ｐ＿（５３）有关，采用单克隆抗体免疫荧光技术对２５例喉癌标本进行了Ｐ（５３）基因表达蛋白定量测定，结果显示，２５例喉癌中有１７例荧光染色阳性，即喉癌中Ｐ（５３）蛋白过度表达为６８．０％（１７／２５），与正常组织相比差异显著（Ｐ＜０．０１），吝期喉癌Ｐ（５３）蛋白表达情况无明显差异（Ｐ＞０．０５）。这说明Ｐ（５３）基因异常导致表达蛋白的堆积是喉癌发生发展中的重要机制之一。 In order to confirm the occurrence and development of laryngeal carcinoma and anti-oncogene P_ (53), monoclonal antibody immunofluorescence technique was used to quantify P (53) gene expression in 25 laryngeal carcinoma specimens. The results showed that in 25 laryngeal carcinoma There were 17 cases of positive fluorescence staining, P (53) protein overexpression was 68.0% (17/25) in laryngeal carcinoma, which was significantly different from the normal tissue (P <0.01) 53) protein expression no significant difference (P> 0.05). This shows that P (53) gene abnormalities lead to the accumulation of the expressed protein is one of the important mechanisms in the development of laryngeal carcinoma.