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主要探讨康定柳(Salix paraplesia)在水培条件下对铯胁迫(133Cs+浓度0、50和200μmol/L)的生理生化响应和富集修复能力。研究结果表明:铯胁迫造成了植物的过氧化损伤,且与胁迫浓度呈正相关;植物通过提高渗透调节能力(Pro)和抗氧化能力(SOD、POD和CAT)应对铯胁迫,但高浓度铯胁迫却显著抑制了植株抗氧化系统的作用。从植物光合荧光指标和叶绿素含量来看,低浓度铯能促进植物叶绿素合成且对光合能力影响不大,而高浓度铯胁迫则抑制了植物叶绿素的合成及光合能力。此外,与对照相比,低浓度铯胁迫能够显著促进植株的茎长,而高浓度铯胁迫则显著抑制了植株的生物量、根长和茎长,对植株生长发育的抑制作用明显。康定柳对水体中铯的富集量和富集系数均表现为根>茎>叶,铯主要富集在植株根部,且在高处理浓度下,植株富集量最高达到2.235 8 mg/g DW。表明康定柳对水体中铯的污染具有较好的耐受性和修复潜力。
The physiological and biochemical responses of Salix paraplesia to cesium stress (133Cs + 0, 50 and 200 μmol / L) under hydroponic culture were investigated. The results showed that: cesium stress caused the peroxidation injury of plants, and had a positive correlation with stress concentration. Plants responded to cesium stress by increasing osmotic adjustment ability (Pro) and antioxidant capacity (SOD, POD and CAT), but high concentration cesium stress But significantly inhibited the role of plant antioxidant system. From the photosynthetic fluorescence index and chlorophyll content of plants, low concentrations of cesium can promote plant chlorophyll synthesis and have little effect on photosynthetic capacity, while high concentrations of cesium stress inhibit plant chlorophyll synthesis and photosynthetic capacity. In addition, compared with the control, low concentration of cesium stress can significantly promote plant stem length, while high concentration of cesium stress significantly inhibited the plant biomass, root length and stem length, the plant growth and development inhibition was obvious. The concentration and enrichment coefficient of cesium in the water body of Kangdingliu were all root> stem> leaf, and cesium was mainly concentrated in the roots of the plants. Under the high concentration, the highest concentration of plant was 2.235 8 mg / g DW . It shows that Kangdingliu has better tolerance and remediation potential to the pollution of cesium in water.